PROTEIN MICROARRAY CORE

 

Protein Microarray Core

Philip Felgner, University of California, Irvine

Abstract:
The goal of the Protein Micro-Array Core is to provide investigators with a high throughput genome cloning platform from which protein microarrays can be made for discovery of vaccine and serodiagnostic antigens. The technology to make whole proteome microarrays is well established, and to date the Core has fabricated arrays containing 16,000 proteins from 20 different infectious agents. Antigens identified by an array can be purified and translated to other accessible serodiagnostic platforms, such as dot arrays in the bottom of 96 well plates (multiplex ELISA) or for the development of subunit vaccines. The Core also offers probing and scanning, protein purification, fabrication of immunostrips /multiplex ELISAs (macroarrays in 96 well plates), and a full statistical analysis through the UCI Institute for Genomics and Bioinformatics. The core aims to expand on these capabilities by developing low cost DNA-based genotyping and high throughput T cell antigen discovery.